Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vivo and In Vitro Effects of a ClpP-Activating Antibiotic against Vancomycin-Resistant Enterococci

doi: 10.1128/AAC.00424-18

Figure Lengend Snippet: Time-kill curves for stationary-phase E. faecalis V583 (VRE) with antibiotics alone (A) or in combination (B). Data for combinations with ADEP4 are shown with dotted lines. Antibiotics used were ampicillin (AMP), ciprofloxacin (CIP), daptomycin (DAP), gentamicin (GEN), linezolid (LZD), oritavancin (ORI), rifampin (RIF), and tigecycline (TGC). Error bars represent standard deviations. The x axis is at the limit of detection.

Article Snippet: E. faecalis strain V583 was used for the in vitro stationary-phase and biofilm killing experiments as well as the in vivo peritoneal septicemia model. Bacterial strains were obtained through the ATCC or BEI Resources or from academic labs (see Table S1 in the supplemental material).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vivo and In Vitro Effects of a ClpP-Activating Antibiotic against Vancomycin-Resistant Enterococci

doi: 10.1128/AAC.00424-18

Figure Lengend Snippet: Killing of mature E. faecalis V583 (VRE) biofilms by antibiotics alone or in combination with ADEP4 after 24, 48, or 72 h. Antibiotics used were ampicillin (AMP), ciprofloxacin (CIP), daptomycin (DAP), gentamicin (GEN), linezolid (LZD), oritavancin (ORI), rifampin (RIF), and tigecycline (TGC). Error bars represent standard deviations. The x axis is at the limit of detection.

Article Snippet: E. faecalis strain V583 was used for the in vitro stationary-phase and biofilm killing experiments as well as the in vivo peritoneal septicemia model. Bacterial strains were obtained through the ATCC or BEI Resources or from academic labs (see Table S1 in the supplemental material).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vivo and In Vitro Effects of a ClpP-Activating Antibiotic against Vancomycin-Resistant Enterococci

doi: 10.1128/AAC.00424-18

Figure Lengend Snippet: In vivo efficacies of ADEP4 alone and in combination with ampicillin (AMP) against murine peritonitis caused by E. faecalis V583 (VRE) infection. ADEP4 was dosed at 80 mg/kg. AMP was dosed at two concentrations: 50 mg/kg (50) and 100 mg/kg (100). Data points are shown, and averages and standard deviations are indicated by horizontal bars. *, P < 0.001 compared to the vehicle group (one-way ANOVA with Tukey's multiple-comparison test). For comparisons between the indicated groups (one-way ANOVA with Tukey's multiple-comparison test), statistical significance is indicated as follows: **, P < 0.01; ***, P < 0.001; and ****, P < 0.0001.

Article Snippet: E. faecalis strain V583 was used for the in vitro stationary-phase and biofilm killing experiments as well as the in vivo peritoneal septicemia model. Bacterial strains were obtained through the ATCC or BEI Resources or from academic labs (see Table S1 in the supplemental material).

Techniques: In Vivo, Infection, Comparison

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vivo and In Vitro Effects of a ClpP-Activating Antibiotic against Vancomycin-Resistant Enterococci

doi: 10.1128/AAC.00424-18

Figure Lengend Snippet: V583 MICs and treatment concentrations for in vitro experiments

Article Snippet: E. faecalis strain V583 was used for the in vitro stationary-phase and biofilm killing experiments as well as the in vivo peritoneal septicemia model. Bacterial strains were obtained through the ATCC or BEI Resources or from academic labs (see Table S1 in the supplemental material).

Techniques: In Vitro, Glycoproteomics